An Introduction to Genetic Engineering by Dr Desmond S. T. Nicholl

By Dr Desmond S. T. Nicholl

Des Nicholl provides a brand new, absolutely revised, and increased variation of his renowned undergraduate-level textbook. The ebook keeps the various gains of the unique version and nonetheless deals a concise technical advent to the topic of genetic engineering. it truly is divided into 3 major sections: uncomplicated molecular biology, equipment of gene manipulation, and sleek functions of genetic engineering. functions coated within the booklet comprise genomics, protein engineering, gene treatment, cloning, transgenic animals and vegetation and bioethics. An creation to Genetic Engineering is key examining for undergraduate scholars of biotechnology, genetics, molecular biology, and biochemistry.

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This problem is magnified at each stage of the process, because losses mean that the amount of material usually diminishes after each step. One way of tracing the material is to label the nucleic acid with a radioactive molecule (usually a deoxynucleoside triphosphate (dNTP), labelled with 3H or 32P), so that portions of each reaction may be counted in a scintillation counter to determine the amount of nucleic acid present. A second application of radiolabelling is in the production of highly radioactive nucleic acid molecules for use in hybridisation experiments.

0% (w/v). On cooling, the agarose sets to form the gel. Agarose gels are usually run in the apparatus shown in Fig. 5. 6), as the pore size is smaller than that achieved with agarose. 1. 1. 4% agarose 4% acrylamide 10% acrylamide 20% acrylamide 50 000 to 1000 20 000 to 300 6000 to 300 1000 to 100 500 to 25 50 to 1 Source: From Schleif and Wensink (1981). Practical Methods in Molecular Biology, Springer-Verlag, New York. Reproduced with permission. and applying a potential difference across it. This is maintained until a marker dye (usually bromophenol blue, added to the sample prior to loading) reaches the end of the gel.

There is the question of safety, as some high-energy radioisotopes are potentially hazardous. Thus the operator must be aware of the risks, and take appropriate precautions when using radioactive isotopes. There are also strict requirements for the disposal of radioactive waste materials. Thus there is growing use made of detection methods that use non-radioactive methods – examples include the use of fluorescent labels in DNA sequencing. We will examine some of these alternative methods later in this book.

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