By Arvid B. Maunsbach, Björn A. Afzelius
This finished reference illustrates optimum education equipment in organic electron microscopy in comparison with universal methodological difficulties. not just will the fundamental methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be offered, however the authors additionally activity to demonstrate extra really good strategies equivalent to detrimental staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted snapshot analysis.
- Authored via the major leaders within the organic electron microscopy field
- Illustrates either optimum and suboptimal or artifactual ends up in numerous electron microscopy disciplines
- Introduces scholars on the best way to learn and interpret electron micrographs
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Extra resources for Biomedical electron microscopy: illustrated methods and interpretations
The temperature of glutaraldehyde fixatives has much less influence on tissue fine structure than that of osmium tetroxide fixatives, except that microtubules seem less well preserved by cold glutaraldehyde fixatives. 2. FIXATIVES 41 42 2. FIXATIVES 6. 2. This mixture corresponds to half-strength "Karnovsky's fixative" (Karnovsky, 1965). The total osmolality of this fixative is about 1000 mOsm/ kg H20. Postfixation was performed with 1% osmium tetroxide in cacodylate buffer, embedding in Epon, and staining with uranyl acetate and lead citrate.
FIXATIVES 51 52 1 1. Osmium Tetroxide-Potassium 2. 5% potassium ferrocyanide. The tissue was embedded in Epon, and the sections were stained with uranyl acetate and lead citrate. • 115,000. 5% potassium ferrocyanide. The tissue was embedded in Epon, and the sections were stained with uranyl acetate and lead citrate. • 50,000. Potassium ferrocyanide added to the osmium tetroxide probably forms a stable complex (Karnovsky, 1971). This method renders certain cellular components, notably membranes, microtubules, and filaments, more electron dense than after postfixation in only osmium tetroxide, and the triple-layered appearance of the cytoplasmic membranes becomes accentuated (Fig.
Luft, J. H. (1956). Permanganate: A new fixative for electron microscopy. J. Biophys. Biochem. Cytol. 2, 799-801. Luft, J. H. (1971). Ruthenium red and violet. I. Chemistry, purification, methods of use for electron microscopy, and mechanisms of action. Anat. Rec. 171, 347-368. Maunsbach, A. B. (1966a). The influence of different fixatives and fixation methods on the ultrastructure of rat kidney proximal tubule cells. I. Comparison of different perfusion fixation methods and of glutaraldehyde, formaldehyde and osmium tetroxide fixatives.