DNA Viruses: Methods and Protocols by Feng Wang-Johanning, Gary L. Johanning (auth.), Paul M.

By Feng Wang-Johanning, Gary L. Johanning (auth.), Paul M. Lieberman (eds.)

A compendium of quite simply reproducible and novel tips on how to manage DNA viruses and represent their diverse organic homes. The authors emphasize concepts for viral detection and genetics, but additionally contain tools for constitution selection, gene expression, replication, pathogenesis, complicated mobile types, recombinant genetics, and computational/systems techniques. Wide-ranging and hugely functional, DNA Viruses: equipment and Protocols will stimulate new instructions in virology study with its novel innovations for engineering viral vectors in gene remedy, and its complicated techniques for detecting viruses in human disorder.

Show description

Read Online or Download DNA Viruses: Methods and Protocols PDF

Best genetics books

The Expression of the Emotions in Man and Animals

The most well-liked of Darwin's books in the course of his lifetime, in a fantastically illustrated new edition

that includes dozens of images from Darwin's unique booklet, this edition-issued to commemorate the 2 hundredth anniversary of Darwin's birth-makes this vintage research newly obtainable to trendy readers. released in 1872, The Expression of the feelings in guy and Animals was once a publication on the very center of Darwin's learn pursuits. Darwin's major aim was once to illustrate the ability of his theories for explaining the beginning of our such a lot loved human traits, morality and mind. The paintings engages many of the toughest questions within the evolution debate, and it exhibits the ever-cautious Darwin at his boldest.

Chaptered EPUB
Appears to be retail

Here's How: Apply to American Colleges and Universities

Aimed toward in another country scholars looking a faculty schooling within the usa, this publication covers each step within the management approach, together with getting the necess ary types, taking required exams, and getting ready monetary in formation. '

Genetics: Analysis of Genes and Genomes

Creation to molecular genetics and genomics -- DNA constitution and DNA manipulation -- Transmission genetics : the main of segregation -- Genes and chromosomes -- Genetic linkage and chromosome mapping -- Molecular biology of DNA replication and recombination -- Molecular biology of DNA replication and recombination -- Molecular mechanisms of mutation and DNA fix -- Molecular association of chromosomes -- Human karyotypes and chromosome habit -- Genetics of micro organism and their viruses -- Molecular biology of gene expression -- Molecular mechanisms of gene rules -- Genetic engineering and genomics -- Genetic keep watch over of improvement -- Molecular genetics of the phone cycle and melanoma -- Extranuclear inheritance -- inhabitants genetics and evolution -- The genetic foundation of advanced inheritance

Primary and Stem Cells: Gene Transfer Technologies and Applications

This publication describes easy mobilephone engineering equipment, emphasizing stem mobilephone purposes, and use of the genetically converted stem cells in mobilephone remedy and drug discovery. jointly, the chapters introduce and provide insights on new strategies for engineering of stem cells and the supply of transgenes into stem cells through a number of viral and non-viral structures.

Additional resources for DNA Viruses: Methods and Protocols

Sample text

A yet undefined latency type with expression of EBER-1/2, EBNA-1, LMP-2a,b, BARTs, and BARF-1 is found in EBV-positive gastric carcinoma (5). BARF-1 mRNA expression is also detected in NPC and appears to be a carcinoma-specific marker not expressed in EBV-positive lymphomas (6,7). However, because BARF-1 mRNA is nonspliced, specific precautions are needed for its detection, as described in refs. 5–7. This chapter describes a sensitive reverse transcription polymerase chain reaction (RT-PCR) assay for determination of EBV latent RNA expression profiles in tumor biopsies or smears, isolated or cultured cells, and unfractionated whole blood.

Measure activity of wash buffer after the last washing step. If this is still active, additional washing steps may be performed until no activity is detected. 34 Stevens, Brink, and Middeldorp Fig. 1. Autoradiograph showing relative sensitivity of multiprimed EBV RT-PCR analysis. A dilution series of 1–100,000 EBV-positive JY cells was made in a whole blood DNA background, originating from an EBV-negative donor. JY is a lymphoblastoid cell line that expresses EBNA-1 mainly from the C/W promotor and shows limited ZEBRA expression in <1% of the cells.

G. BARTs (primers A3 and A4). h. ZEBRA (primers Z1 and Z2). i. The cellular housekeeping gene U1A snRNP (primers U1A1 and U1A2). 2 µL AmpliTaq DNA polymerase. Multiprimed EBV RT-PCR 33 4. Cycle the samples in a PCR device using the following PCR program: 4 min at 95°C; 40 cycles of 1 min at 95°C, 1 min at 55°C, and 1 min at 72°C; 7 min at 72°C, and finally a hold at 4°C (see Note 15). 4. 1. Electrophoretic Separation of PCR Products and Blotting to Nylon 1. 5% agarose gel electrophoresis in TBE buffer for 1–2 h at approx 100 mA.

Download PDF sample

Rated 4.57 of 5 – based on 33 votes